Additive-026
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ID： |
C9386A5E9AAA435BA2AFEB93F268A08E |
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文件大小(MB)： |
0.1 |
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页数： |
4 |
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文件格式： |
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日期： |
2014-9-11 |
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购买： |
文本摘录（文本识别可能有误，但文件阅览显示及打印正常，pdf文件可进行文字搜索定位）：
AMMONIUM SALTS of PHOSPHATIDIC ACID,Prepared at the 55th JECFA (2000) and published in FNP 52 Add 8 (2000), superseding tentative specifications prepared at the 17th JECFA (1973) and published in FNP 4 (1978) and in FNP 52 (1992). An ADI of 0-30 mg/kg bw was established at the 18th JECFA (1974).,SYNONYMS,Ammonium phosphatides, Emulsifier YN, Mixed ammonium salts of phosphorylated glycerides; INS No. 442,DEFINITION,The product consists essentially of a mixture of the ammonium compounds of phosphatidic acids derived from the edible fat (usually partially hardened rapeseed oil). A mono- or diglyceride moiety may be attached to phosphorus. Moreover, two phosphorus esters may be linked together as phosphatidyl phosphatides. The product is prepared by glycerolysis of the fat, followed by phosphorylation with phosphorus pentoxide, and neutralization with ammonia.,The article of commerce may be further specified as to water content, hexane-insoluble matter, inorganic hexane-insoluble matter, pH value and triglyceride content.,Structural formula (approximate composition),where R may be a mono- or di-glyceride moiety,Assay,The phosphorus content is not less than 3.0% and not more than 3.4% by weight; the ammonium N content is not less than 1.2% and not more than 1.5%.,DESCRIPTION,Unctuous semisolid,FUNCTIONAL USES,Emulsifier,CHARACTERISTICS,IDENTIFICATION,Solubility (Vol. 4),Insoluble in water, partially soluble in ethanol and in acetone, soluble in fats,Test for phosphate,Passes tests,See description under TESTS,Test for fatty acid,Passes tests,See description under TESTS,Test for glycerol (Vol. 4),Passes test,PURITY,Lead (Vol. 4),Not more than 2 mg/kg,Determine using an atomic absorption technique appropriate to the specified level. The selection of sample size and method of sample preparation may be based on the principles of the method described in Volume 4, “Instrumental Methods.”,TESTS,IDENTIFICATION TESTS,Test for phosphate,Ignite 1 g of the sample with 2 g of anhydrous sodium carbonate. Cool and dissolve the residue in 5 ml of water and 5 ml of nitric acid. Add 5 ml of ammonium molybdate TS and heat to boiling. A yellow precipitate is obtained.,Test for fatty acid,Reflux 1 g of the product for 1 h with 25 ml of 0.5 N ethanolic potassium fatty acid hydroxide. Ammonia is evolved from the end of the reflux condenser, recognizable by its odour and by its reaction on moist, red litmus paper. On cooling the residue to 0o, a precipitate of potassium soap is obtained.,METHOD OF,ASSAY,Determination of phosphorus,Absorption spectrophotometry,Reagents and working solutions,Sulfuric acid: sp gr 1.84,Nitric acid: sp gr 1.42,Perchloric acid: 60%, sp gr 1.54,Vanadate-molybdate solution: Separately dissolve in water 20 g of ammonium molybdate and 1 g of ammonium vanadate. Mix the two solutions, add 140 ml of concentrated nitric acid and dilute to 1000 ml with water. Mix well.,Standard phosphate solution: Stock solution: Dissolve 3.8346 g of potassium dihydrogen phosphate, previously dried at 110o, in water and dilute to 1000 ml; 1 ml of this solution = 2.0 mg P2O5.,Standard phosphate working solution: Transfer 50.0 ml of the standard phosphate solution (1ml = 2.0 mg P2O5) to a 500 ml volumetric flask and dilute to volume with water and mix well. 1 ml of standard phosphate working solution = 0.2 mg equivalents of P2O5,Procedure,Weigh accurately 1.5 to 1.6 g of a representative sample into a small,glass capsule and transfer to a 300-ml Kjeldahl flask containing 5 ml of sulfuric acid and 10 ml of nitric acid. Heat the flask, gently at first, with continual swirling, and later more strongly over a bare flame. Add further measured amounts of nitric acid from time to time, cooling the flask prior to addition, and continue the heating until the stage where the digest is clear and assumes a golden colour. Cool, add 5 ml of 60% perchloric acid and continue the oxidation until white acid fumes form in the flask. Cool again and add 5 ml of water and continue heating until white fumes are again driven off. Cool, dilute carefully with water, cool again and transfer quantitatively to a 500-ml volumetric flask. Dilute to volume with water and mix well (Test solution).,Carry out a blank digestion exactly as above but omit the sample and use the same volume of acid as required to wet oxidize the sample (Blank digest solution).,Into separate 100-ml volumetric flasks, add by burette:,(a) 25.0 ml of Standard phosphate working solution (= 5.0 mg P2O5),(b) 30.0 ml of Standard phosphate working solution (= 6.0 mg P2O5),(c) a 25 ml aliquot of the test solution which will contain the equivalent of between 5 and 6 mg……
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